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Objective To analyze anticancer activity of an ethyl acetate extract of endophytic fungi isolated from soursop leaf (Annona muricata L.). Methods Anticancer activity of fungal extracts was determined by observing its toxicity against MCF-7 (Michigan Cancer Foundation-7) cells in vitro by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay method. At an extract concentration of 100 μg/mL, 4 isolates out of 12 showed high activity against the cancer cell growth. The four isolates were then selected for further IC
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OBJECTIVE@#To characterize abundance of leaf litter actinomycetes from Ternate Island and to assess the antifungal activity of actinomycetes isolates against Candida albicans, Saccharomyces cerevisiae (S. cerevisiae), and Aspergillus niger.@*METHODS@#Actinomycetes were isolated from leaf litter of Durio species, Syzygium aromaticum, Piper betle, Myristica fragrans, or Pandanus species and unknown plants. Actinomycetes isolates were cultured in a liquid medium. Bioactive compounds were extracted and tested against fungal using Beury-Kirby method with modification. Minimum inhibitor concentration and cell leakages were conducted. Actinomycetes that produced the highest antifungal activity were indentified using molecular sequence data in 16S rRNA gene.@*RESULTS@#Out of 50 selected isolates, two isolates MG-500-1-4 and SR-2-2 has highest activity against S. cerevisiae. Concentration of material containing nucleic acids, proteins, Ca(+) and K(+) ions and morphological observations indicated that extracts of MG-500-1-4 and SR-2-2 caused cell leakage and invagination of S. cerevisiae cells. Based on 16S rRNA gene identification, MG-500-1-4 and SR-2-2 isolates are similar to Streptomyces misakiensis and Streptomyces tricolor respectively.@*CONCLUSIONS@#Ternate Island contains interesting biodiversity of actinomycetes that has potential use in agriculture, fisheries, and human health to reduce problem of fungal pathogen.
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Objective: To characterize abundance of leaf litter actinomycetes from Ternate Island and to assess the antifungal activity of actinomycetes isolates against Candida albicans, Saccharomyces cerevisiae (S. cerevisiae), and Aspergillus niger. Methods: Actinomycetes were isolated from leaf litter of Durio species, Syzygium aromaticum, Piper betle, Myristica fragrans, or Pandanus species and unknown plants. Actinomycetes isolates were cultured in a liquid medium. Bioactive compounds were extracted and tested against fungal using Beury-Kirby method with modification. Minimum inhibitor concentration and cell leakages were conducted. Actinomycetes that produced the highest antifungal activity were indentified using molecular sequence data in 16S rRNA gene. Results: Out of 50 selected isolates, two isolates MG-500-1-4 and SR-2-2 has highest activity against S. cerevisiae. Concentration of material containing nucleic acids, proteins, Ca